|
KMID : 0894519970010010029
|
|
Development & Reproduction
1997 Volume.1 No. 1 p.29 ~ p.36
|
|
|
|
|
장윤정
장영진/임한규
|
|
|
Abstract
|
|
|
|
Experiments were performed to study the effects of diluents, cryoprotectant, equilibration time, thawing temperature and addition of BSA and egg yolk. Among the various diluents, Alsever's solution was the best for sperm cryopreservation. A combination of Alsever's solution and 15% ethylene glycol showed the better results than others did. Sperm activity indection and survival rate gradually decreased with the equilibration time. The appropriate thawing temperature was 30 ±1?C. These results indicate that sperm cryopreservation methods can be developed in tiger puffer.
|
|
|
KEYWORD
|
|
|
Tiger puffer (Takifugu rubripes), Sperm cryopreservation, Diluent, Cryoprotectant, Equili-bration time, Thawing temperature
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|